Effect of intra- and inter-tumoral heterogeneity on molecular characteristics of primary IDH-wild type glioblastoma revealed by single-cell analysis.

ABSTRACT:

Aims

To reveal the effects of intra- and inter-tumoral heterogeneity on characteristics of primary IDH-wild type glioblastoma cells.

Methods

Single-cell RNA-seq data were acquired from the GEO database, and bulk sample transcriptome data were downloaded from the TCGA database with clinical information. Neoplastic subtype and glioma stem-like cells (GSCs) were identified by matching 5000 random virtual samples based on ssGSEA. CNV was inferred to compare the heterogeneity among patients and subtypes by infercnv. Transition direction was inferred by RNA velocity, and lineage trajectory was inferred by monocle. Regulon network of cells was analyzed by SCENIC, and cell communication was identified by CellPhoneDB.

Results

Glioblastoma (GBM) cells could be divided into four

SUBMITTER: Xiong Z 

PROVIDER: S-EPMC7415209 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature