Wounding Induces Facultative Opn5-Dependent Circadian Photoreception in the Murine Cornea.

ABSTRACT:

Purpose

Autonomous molecular circadian clocks are present in the majority of mammalian tissues. These clocks are synchronized to phases appropriate for their physiologic role by internal systemic cues, external environmental cues, or both. The circadian clocks of the in vivo mouse cornea synchronize to the phase of the brain's master clock primarily through systemic cues, but ex vivo corneal clocks entrain to environmental light cycles. We evaluated the underlying mechanisms of this difference.

Methods

Molecular circadian clocks of mouse corneas were evaluated in vivo and ex vivo for response to environmental light. The presence of opsins and effect of genetic deletion of opsins were evaluated for influence on circadian photoresponses. Opn5-expressing cells were identified u

SUBMITTER: Diaz NM 

PROVIDER: S-EPMC7415322 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature