Metformin Corrects Abnormal Circadian Rhythm and Kir4.1 Channels in Diabetes.

ABSTRACT:

Purpose

Diabetic retinopathy (DR) is a leading cause of visual impairment. Müller cells in DR are dysfunctional due to downregulation of the inwardly rectifying potassium channel Kir4.1. Metformin, a commonly used oral antidiabetic drug, is known to elicit its action through 5' adenosine monophosphate-activated protein kinase (AMPK), a cellular metabolic regulator; however, its effect on Kir4.1 channels is unknown. For this study, we hypothesized that metformin treatment would correct circadian rhythm disruption and Kir4.1 channel dysfunction in db/db mice.

Methods

Metformin was given orally to db/db mice. Wheel-running activity, retinal levels of Kir4.1, and AMPK phosphorylation were determined at study termination. In parallel, rat retinal Müller cell line (rMC-1) cells we

SUBMITTER: Alex A 

PROVIDER: S-EPMC7415324 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature