The potential use of the Penicillium chrysogenum antifungal protein PAF, the designed variant PAF^opt and its γ-core peptide Pγ^opt in plant protection.

ABSTRACT: The prevention of enormous crop losses caused by pesticide-resistant fungi is a serious challenge in agriculture. Application of alternative fungicides, such as antifungal proteins and peptides, provides a promising basis to overcome this problem; however, their direct use in fields suffers limitations, such as high cost of production, low stability, narrow antifungal spectrum and toxicity on plant or mammalian cells. Recently, we demonstrated that a Penicillium chrysogenum-based expression system provides a feasible tool for economic production of P. chrysogenum antifungal protein (PAF) and a rational designed variant (PAF^opt ), in which the evolutionary conserved γ-core motif was modified to increase antifungal activity. In the present study, we report for the first time that

SUBMITTER: Toth L 

PROVIDER: S-EPMC7415367 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature