Ewing sarcoma family of tumors-derived small extracellular vesicle proteomics identify potential clinical biomarkers.

ABSTRACT:

Purpose

Ewing Sarcoma Family of Tumors (ESFT), the second most common pediatric osseous malignancy, are characterized by the pathognomonic chromosomal EWS-ETS translocation. Outside of tumor biopsy, no clinically relevant ESFT biomarkers exist. Additionally, tumor burden assessment at diagnosis, monitoring of disease responsiveness to therapy, and detection of disease recurrence are limited to radiographic imaging. To identify new, clinically relevant biomarkers we evaluated the proteome of a subset of ESFT-derived small extracellular vesicles (sEVs).

Materials and methods

We performed the first high quality proteomic study of ESFT-derived sEVs from 5 ESFT cell lines representing the most common EWS-ETS fusion types and identified 619 proteins composing the cor

SUBMITTER: Samuel G 

PROVIDER: S-EPMC7415402 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature