AURKB Promotes the Metastasis of Gastric Cancer, Possibly by Inducing EMT.

ABSTRACT:

Aim

To investigate the function of Aurora kinase B (AURKB) in gastric cancer (GC).

Methods

Immunohistochemistry was used to assay the expression of AURKB in 50 pairs of GC and adjacent tissues, and qRT-PCR was conducted to test AURKB expression in normal gastric epithelial and GC cell lines. Two segments of small interference RNAs (siRNAs) targeting AURKB were synthesized and inserted into GV248 lentivirus vector. After transfected with LV-AURKB-RNAis, CCK8, wound healing, transwell and flow cytometric assays were performed to determine the influence of silencing AURKB on cell proliferation, invasion, migration, cell cycles and apoptosis of GC cells, and the expression of EMT (epithelial-mesenchymal transition)-related markers was demonstrated by Western

SUBMITTER: Wang Z 

PROVIDER: S-EPMC7415439 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature