A Chromatin Accessibility Atlas of the Developing Human Telencephalon.

ABSTRACT: To discover regulatory elements driving the specificity of gene expression in different cell types and regions of the developing human brain, we generated an atlas of open chromatin from nine dissected regions of the mid-gestation human telencephalon, as well as microdissected upper and deep layers of the prefrontal cortex. We identified a subset of open chromatin regions (OCRs), termed predicted regulatory elements (pREs), that are likely to function as developmental brain enhancers. pREs showed temporal, regional, and laminar differences in chromatin accessibility and were correlated with gene expression differences across regions and gestational ages. We identified two functional de novo variants in a pRE for autism risk gene SLC6A1, and using CRISPRa, demonstrated that this pRE regulat

SUBMITTER: Markenscoff-Papadimitriou E 

PROVIDER: S-EPMC7415678 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature