KLRG1⁺ Memory CD8 T Cells Combine Properties of Short-Lived Effectors and Long-Lived Memory.

ABSTRACT: CD8 effector T cells with a CD127^hi KLRG1^- phenotype are considered precursors to the long-lived memory pool, whereas KLRG1⁺CD127^low cells are viewed as short-lived effectors. Nevertheless, we and others have shown that a KLRG1⁺CD127^low population persists into the memory phase and that these T cells (termed long-lived effector cells [LLEC]) display robust protective function during acute rechallenge with bacteria or viruses. Whether these T cells represent a true memory population or are instead a remnant effector cell population that failed to undergo initial contraction has remained unclear. In this study, we show that LLEC from mice express a distinct phenotypic and transcriptional signature that shares characteristics of both early effectors and long-lived memory cells. We also find that in contrast to KLRG1⁺ effector cells, LLEC undergo homeostatic proliferation and are not critically dependent on IL-15 for their maintenance. Furthermore, we find that LLEC are predominantly derived from KLRG1⁺ effector cells when isolated at day 12 of the response. Our work challenges the concept that the KLRG1⁺CD127^low population is dominated by short-lived cells and shows that KLRG1 downregulation is not a prerequisite to become a long-lived protective memory T cell.

SUBMITTER: Renkema KR 

PROVIDER: S-EPMC7415731 | biostudies-literature |

REPOSITORIES: biostudies-literature