Epigenetic regulator function through mouse gastrulation.

ABSTRACT: During ontogeny, proliferating cells become restricted in their fate through the combined action of cell-type-specific transcription factors and ubiquitous epigenetic machinery, which recognizes universally available histone residues or nucleotides in a context-dependent manner^1,2. The molecular functions of these regulators are generally well understood, but assigning direct developmental roles to them is hampered by complex mutant phenotypes that often emerge after gastrulation^3,4. Single-cell RNA sequencing and analytical approaches have explored this highly conserved, dynamic period across numerous model organisms^5-8, including mouse^9-18. Here we advance these strategies using a combined zygotic perturbation and single-cell RNA-sequencing pla

SUBMITTER: Grosswendt S 

PROVIDER: S-EPMC7415732 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature