Transactivation of Sus1 and Sus2 by Opaque2 is an essential supplement to sucrose synthase-mediated endosperm filling in maize.

ABSTRACT: The endosperm-specific transcription factor Opaque2 (O2) acts as a central regulator for endosperm filling, but its functions have not been fully defined. Regular o2 mutants exhibit a non-vitreous phenotype, so we used its vitreous variety Quality Protein Maize to create EMS-mutagenesis mutants for screening o2 enhancers (oen). A mutant (oen1) restored non-vitreousness and produced a large cavity in the seed due to severely depleted endosperm filling. When oen1 was introgressed into inbred W64A with a normal O2 gene, the seeds appeared vitreous but had a shrunken crown. oen1 was determined to encode Shrunken1 (Sh1), a sucrose synthase (SUS, EC 2.4.1.13). Maize contains three SUS-encoding genes (Sh1, Sus1, and Sus2) with Sh1 contributing predominantly to the endosperm. We determined SUS act

SUBMITTER: Deng Y 

PROVIDER: S-EPMC7415785 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature