METTL14 aggravates endothelial inflammation and atherosclerosis by increasing FOXO1 N6-methyladeosine modifications.

ABSTRACT: Aims: The N6-methyladenosine (m⁶A) modification plays an important role in various biological processes, but its role in atherosclerosis remains unknown. The aim of this study was to investigate the role and mechanism of m⁶A modification in endothelial cell inflammation and its influence on atherosclerosis development. Methods: We constructed a stable TNF-α-induced endothelial cell inflammation model and assessed the changes in the expression of m⁶A modification-related proteins to identify the major factors involved in this process. The m⁶A-modified mRNAs were identified by methylated RNA immunoprecipitation (RIP) sequencing and forkhead box O1 (FOXO1) was selected as a potential target. Through cytological experiments, we verified

SUBMITTER: Jian D 

PROVIDER: S-EPMC7415798 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature