Fine-Needle Aspiration-Based Patient-Derived Cancer Organoids.

ABSTRACT: Patient-derived cancer organoids hold great potential to accurately model and predict therapeutic responses. Efficient organoid isolation methods that minimize post-collection manipulation of tissues would improve adaptability, accuracy, and applicability to both experimental and real-time clinical settings. Here we present a simple and minimally invasive fine-needle aspiration (FNA)-based organoid culture technique using a variety of tumor types including gastrointestinal, thyroid, melanoma, and kidney. This method isolates organoids directly from patients at the bedside or from resected tissues, requiring minimal tissue processing while preserving the histologic growth patterns and infiltrating immune cells. Finally, we illustrate diverse downstream applications of this technique includi

SUBMITTER: Vilgelm AE 

PROVIDER: S-EPMC7415927 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature