New analysis pipeline for high-throughput domain-peptide affinity experiments improves SH2 interaction data.

ABSTRACT: Protein domain interactions with short linear peptides, such as those of the Src homology 2 (SH2) domain with phosphotyrosine-containing peptide motifs (pTyr), are ubiquitous and important to many biochemical processes of the cell. The desire to map and quantify these interactions has resulted in the development of high-throughput (HTP) quantitative measurement techniques, such as microarray or fluorescence polarization assays. For example, in the last 15 years, experiments have progressed from measuring single interactions to covering 500,000 of the 5.5 million possible SH2-pTyr interactions in the human proteome. However, high variability in affinity measurements and disagreements about positive interactions between published data sets led us here to reevaluate the analysis methods and r

SUBMITTER: Ronan T 

PROVIDER: S-EPMC7415975 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature