Highly Sensitive Amperometric α-Ketoglutarate Biosensor Based on Reduced Graphene Oxide-Gold Nanocomposites.

ABSTRACT: Herein, a rapid and highly sensitive amperometric biosensor for the detection of α-ketoglutarate (α-KG) was constructed via an electrochemical approach, in which the glutamate dehydrogenase (GLUD) was modified on the surface of reduced graphene oxide-gold nanoparticle composite (rGO-Aunano composite). The rGO-Aunano composite was one-step electrodeposited onto glassy carbon electrode (GCE) surface and was characterized by scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), and electrochemical techniques. In addition, the rGO-Aunano/GCE was also found to electrocatalyze the oxidation of β-nicotinamide adenine dinucleotide (NADH) at the peak potential of 0.3 V, which was negatively shifted compared with that at bare GCE or Aunano/GCE, illustrating better catalytic

SUBMITTER: Peng G 

PROVIDER: S-EPMC7416262 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature