Probing myeloid cell dynamics in ischaemic heart disease by nanotracer hot-spot imaging.

ABSTRACT: Ischaemic heart disease evokes a complex immune response. However, tools to track the systemic behaviour and dynamics of leukocytes non-invasively in vivo are lacking. Here, we present a multimodal hot-spot imaging approach using an innovative high-density lipoprotein-derived nanotracer with a perfluoro-crown ether payload (¹⁹F-HDL) to allow myeloid cell tracking by ¹⁹F magnetic resonance imaging. The ¹⁹F-HDL nanotracer can additionally be labelled with zirconium-89 and fluorophores to detect myeloid cells by in vivo positron emission tomography imaging and optical modalities, respectively. Using our nanotracer in atherosclerotic mice with myocardial infarction, we observed rapid myeloid cell egress from the spleen and bone marrow by in vivo ¹⁹F-

SUBMITTER: Senders ML 

PROVIDER: S-EPMC7416336 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature