Glutamine uptake and utilization of human mesenchymal glioblastoma in orthotopic mouse model.

ABSTRACT:

Background

Glioblastoma (GBM) are highly heterogeneous on the cellular and molecular basis. It has been proposed that glutamine metabolism of primary cells established from human tumors discriminates aggressive mesenchymal GBM subtype to other subtypes.

Methods

To study glutamine metabolism in vivo, we used a human orthotopic mouse model for GBM. Tumors evolving from the implanted primary GBM cells expressing different molecular signatures were analyzed using mass spectrometry for their metabolite pools and enrichment in carbon 13 (¹³C) after ¹³C-glutamine infusion.

Results

Our results showed that mesenchymal GBM tumors displayed increased glutamine uptake and utilization compared to both control brain tissue and other GBM subtypes. Furthermore,

SUBMITTER: Oizel K 

PROVIDER: S-EPMC7416393 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature