Improved sensitivity of laser-enhanced ¹H^α-¹³C^α-correlation via suppression of C^α-C' scalar-coupling evolution.

ABSTRACT: Low-concentration photochemically induced dynamic polarization (LC-photo-CIDNP) enables the spectroscopic analysis of biomolecules containing the amino acids Trp and Tyr at sub-micromolar concentration in solution. Typical LC-photo-CIDNP pulse sequences involving ¹H-¹³C correlation, however, perform well in the case of aromatic resonances but display a relatively poor signal-to-noise ratio for ¹³C^α and ¹³C^β resonances. Here, we develop a novel pulse sequence denoted as ¹³C perturbation-recovered selective-pulse photo-CINDP enhanced reverse INEPT, or ¹³C PRESPRINT, tailored to the LC-photo-CIDNP analysis of ¹H-¹³C^α pairs. Our method, which is based on full suppression of

SUBMITTER: Yang H 

PROVIDER: S-EPMC7416423 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature