In vitro propagation and DNA barcode analysis of the endangered Silene schimperiana in Saint Katherine protectorate.

ABSTRACT: BACKGROUND:Anthropogenic activity, climate change, pollution, and exploitation of natural resources are some reasons that cause threatening of plant diversity. Silene schimperiana is an endangered plant species in Egypt and is endemic to the high mountain of Saint Katherine Protected Area in southern Sinai. The purpose of the study was the ex situ conservation of Silene schimperiana through in vitro propagation and DNA barcode analysis. RESULTS:To develop an efficient ex situ conservation program of the plant, in vitro propagation protocol has been achieved from shoot tip and stem nodal segment explants of in vitro germinated seedlings. Explants were established in vitro on Murashige and Skoog (MS) medium supplemented with 2.89 μM gibberellic acid (GA3), 1.08 μM α-naphthaleneacetic acid (N

SUBMITTER: Ghareb HE 

PROVIDER: S-EPMC7417468 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature