Regulated expression and function of the GABA_B receptor in human pancreatic beta cell line and islets.

ABSTRACT: G protein-coupled receptors are seven transmembrane signaling molecules that are involved in a wide variety of physiological processes. They constitute a large protein family of receptors with almost 300 members detected in human pancreatic islet preparations. However, the functional role of these receptors in pancreatic islets is unknown in most cases. We generated a new stable human beta cell line from neonatal pancreas. This cell line, named ECN90 expresses both subunits (GABBR1 and GABBR2) of the metabotropic GABA_B receptor compared to human islet. In ECN90 cells, baclofen, a specific GABA_B receptor agonist, inhibits cAMP signaling causing decreased expression of beta cell-specific genes such as MAFA and PCSK1, and reduced insulin secretion. We next demonstrated t

SUBMITTER: Rachdi L 

PROVIDER: S-EPMC7417582 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature