Structural Characterization of TssL from Acinetobacter baumannii: a Key Component of the Type VI Secretion System.

ABSTRACT: The type VI secretion system (T6SS) is a complex molecular nanomachine used by Gram-negative bacteria to deliver diverse effectors into adjacent cells. A membrane complex (MC) anchors this transport system to the bacterial cell wall. One of the proteins forming the MC is TssL, a cytoplasmic protein bound to the inner membrane through a single transmembrane helix. Here, we report the structure of the cytoplasmic N-terminal region of TssL from Acinetobacter baumannii, a bacterium encoding in a single locus a secretion system that is a special case among other T6SSs. The protein structure, consisting of two antiparallel alpha-helical bundles connected by a short loop, reveals several interesting particularities compared with homologous proteins from other organisms. In addition, we demonstrat

SUBMITTER: Ruiz FM 

PROVIDER: S-EPMC7417833 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature