Ageing and genetic background influence anaesthetic effects in a D. melanogaster model of blunt trauma with brain injury^†.

ABSTRACT:

Background

General anaesthetics interact with the pathophysiological mechanisms of traumatic brain injury (TBI). We used a Drosophila melanogaster (fruit fly) model to test the hypothesis that ageing and genetic background modulate the effect of anaesthetics and hyperoxia on TBI-induced mortality in the context of blunt trauma.

Methods

We exposed flies to isoflurane or sevoflurane under normoxic or hyperoxic conditions and TBI, and subsequently quantified the effect on mortality 24 h after injury. To determine the effect of age on anaesthetic-induced mortality, we analysed flies at 1-8 and 43-50 days old. To determine the effect of genetic background, we performed a genome-wide association study (GWAS) analysis on a collection of young inbred, fully sequenced lines.

Resul

SUBMITTER: Schiffman HJ 

PROVIDER: S-EPMC7418562 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature