Modular control of l-tryptophan isotopic substitution via an efficient biosynthetic cascade.

ABSTRACT: Isotopologs are powerful tools for investigating biological systems. We report a biosynthetic-cascade synthesis of Trp isotopologs starting from indole, glycine, and formaldehyde using the enzymes l-threonine aldolase and an engineered β-subunit of tryptophan synthase. This modular route to Trp isotopologs is simple and inexpensive, enabling facile access to these compounds.

SUBMITTER: Thompson CM 

PROVIDER: S-EPMC7418710 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature