Robotic High-Throughput Biomanufacturing and Functional Differentiation of Human Pluripotent Stem Cells.

ABSTRACT: Efficient translation of human induced pluripotent stem cells (hiPSCs) depends on implementing scalable cell manufacturing strategies that ensure optimal self-renewal and functional differentiation. Currently, manual culture of hiPSCs is highly variable and labor-intensive posing significant challenges for high-throughput applications. Here, we established a robotic platform and automated all essential steps of hiPSC culture and differentiation under chemically defined conditions. This streamlined approach allowed rapid and standardized manufacturing of billions of hiPSCs that can be produced in parallel from up to 90 different patient-and disease-specific cell lines. Moreover, we established automated multi-lineage differentiation to generate primary embryonic germ layers and more mature

SUBMITTER: Tristan CA 

PROVIDER: S-EPMC7418713 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature