Analysis of single-cell RNAseq identifies transitional states of T cells associated with hepatocellular carcinoma.

ABSTRACT:

Background

Exhausted T cells and regulatory T cells (Tregs) comprise diverse subsets of tumor immunosuppressive microenvironment that play key roles in tumor progress. Understanding subset diversity in T cells is a critical question for developing cancer immunotherapy.

Methods

A total of 235 specimens from surgical resections of hepatocellular carcinoma (HCC) patients were examined for infiltration of exhausted T cell (Tex) in tumor and adjacent tissue. We conducted deep single-cell targeted immune profiling on CD3⁺ cells collected from tumor tissues, adjacent normal tissues (ANTs) and peripheral blood of HCC patients. Total 10 cell clusters were identified with distinct distributions and characteristics.

Results

We observed transitional differentiation of

SUBMITTER: Yang Y 

PROVIDER: S-EPMC7418813 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature