More cell culture passaged Camelpox virus sequences found resembling those of vaccinia virus.

ABSTRACT:

Background

Camelpox is the most infectious and economically important disease of camelids that causes significant morbidity and mortality rates. Several live attenuated vaccines against Camelpox virus (CMLV) are produced worldwide by passaging field isolates in cell culture. Sequence of a high passage Saudi isolate of CMLV was previously found closely resembled Vaccinia virus (VACV).

Aim

To determine whether other high cell culture passage CMLV isolates are genetically resemble VACV and further to explore the possible mechanism of the resemblance.

Methods

We performed polymerase chain reaction and DNA sequence analysis of A-type inclusion body protein (ATIP), L1R, and open reading frame (ORF) 185 genes on different cell culture passage levels of a field isolate

SUBMITTER: Khalafalla AI 

PROVIDER: S-EPMC7419068 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature