Unexpected cases in field diagnosis of African swine fever virus in Vietnam: The needs consideration when performing molecular diagnostic tests.

ABSTRACT:

Background

The first confirmed case of African swine fever (ASF) in Vietnam was reported officially in February 2019. To date, ASF virus (ASFV) have been detected in 63/63 provinces in Vietnam. Currently, real-time polymerase chain reaction (PCR) is considered to be a powerful tool for viral detection in field samples, including ASFV. However, some recent reports have suggested that mismatches in primer and probe binding regions may directly affect real-time PCR qualification, leading a false-negative result.

Aim

This study aims to further examine a conflicting result obtained from two OIE recommended methods, conventional PCR and real-time PCR, for ASFV detection.

Methods

Two ASF suspected pigs from different provinces in the north of Vietnam were selected for this s

SUBMITTER: Truong AD 

PROVIDER: S-EPMC7419073 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature