Mapping the Segmental Microbiomes in the Human Small Bowel in Comparison with Stool: A REIMAGINE Study.

ABSTRACT:

Background

Most gut microbiome studies have been performed using stool samples. However, the small intestine is of central importance to digestion, nutrient absorption, and immune function, and characterizing its microbial populations is essential for elucidating their roles in human health and disease.

Aims

To characterize the microbial populations of different small intestinal segments and contrast these to the stool microbiome.

Methods

Male and female subjects undergoing esophagogastroduodenoscopy without colon preparation were prospectively recruited. Luminal aspirates were obtained from the duodenum, jejunum, and farthest distance reached. A subset also provided stool samples. 16S rRNA sequencing was performed and analyses were carried out using CLC Genomics Work

SUBMITTER: Leite GGS 

PROVIDER: S-EPMC7419378 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature