HASLR: Fast Hybrid Assembly of Long Reads.

ABSTRACT: Third-generation sequencing technologies from companies such as Oxford Nanopore and Pacific Biosciences have paved the way for building more contiguous and potentially gap-free assemblies. The larger effective length of their reads has provided a means to overcome the challenges of short to mid-range repeats. Currently, accurate long read assemblers are computationally expensive, whereas faster methods are not as accurate. Moreover, despite recent advances in third-generation sequencing, researchers still tend to generate accurate short reads for many of the analysis tasks. Here, we present HASLR, a hybrid assembler that uses error-prone long reads together with high-quality short reads to efficiently generate accurate genome assemblies. Our experiments show that HASLR is not only the fast

SUBMITTER: Haghshenas E 

PROVIDER: S-EPMC7419660 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature