Axonal Extensions along Corticospinal Tracts from Transplanted Human Cerebral Organoids.

ABSTRACT: The reconstruction of lost neural circuits by cell replacement is a possible treatment for neurological deficits after cerebral cortex injury. Cerebral organoids can be a novel source for cell transplantation, but because the cellular composition of the organoids changes along the time course of the development, it remains unclear which developmental stage of the organoids is most suitable for reconstructing the corticospinal tract. Here, we transplanted human embryonic stem cell-derived cerebral organoids at 6 or 10 weeks after differentiation (6w- or 10w-organoids) into mouse cerebral cortices. 6w-organoids extended more axons along the corticospinal tract but caused graft overgrowth with a higher percentage of proliferative cells. Axonal extensions from 10w-organoids were smaller in num

SUBMITTER: Kitahara T 

PROVIDER: S-EPMC7419717 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature