Imaging Transplanted Photoreceptors in Living Nonhuman Primates with Single-Cell Resolution.

ABSTRACT: Stem cell-based transplantation therapies offer hope for currently untreatable retinal degenerations; however, preclinical progress has been largely confined to rodent models. Here, we describe an experimental platform for accelerating photoreceptor replacement therapy in the nonhuman primate, which has a visual system much more similar to the human. We deployed fluorescence adaptive optics scanning light ophthalmoscopy (FAOSLO) to noninvasively track transplanted photoreceptor precursors over time at cellular resolution in the living macaque. Fluorescently labeled photoreceptors generated from a CRX^+/tdTomato human embryonic stem cell (hESC) reporter line were delivered subretinally to macaques with normal retinas and following selective ablation of host photoreceptors using an

SUBMITTER: Aboualizadeh E 

PROVIDER: S-EPMC7419740 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature