Effect of transient scrotal hyperthermia on human sperm: an iTRAQ-based proteomic analysis.

ABSTRACT:

Background

Through this prospective study, we aimed to explore the change of molecular modification after the transient scrotal hyperthermia on human sperm.

Methods

Ten healthy subjects selected with strict screening criteria underwent testicular warming in a 43 °C water bath for 30 min a day for 10 consecutive days. Semen samples were collected 2 weeks before the first heat treatment and 6 weeks after the first heat treatment. Proteins from the samples were labeled with isobaric tags for relative and absolute quantitation and analyzed by two-dimensional liquid chromatography-tandem mass spectrometry.

Results

In contrast to the control, of the 3446 proteins identified, 61 proteins were deregulated: 28 were up-regulated and 33 were down-regulated. Approximately 95% of

SUBMITTER: Wu YQ 

PROVIDER: S-EPMC7422586 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature