Perfusion culture maintained with an air-liquid interface to stimulate epithelial cell organization in renal organoids in vitro.

ABSTRACT:

Background

Organoids derived from induced pluripotent stem (iPS) or embryonic stem (ES) cells have been evaluated as in vitro models of development and disease. However, maintaining these cells under long-term static culture conditions is difficult because of nutrition shortages and waste accumulation. To overcome these issues, perfusion culture systems are required for organoid technology. A system with a stable microenvironment, nutrient availability, and waste removal will accelerate organoid generation. The aim of this study was to develop a novel perfusion system for renal organoids by maintaining the air-liquid interface with a device fabricated using a 3D printer.

Results

Our results revealed slow flow at the organoid cultivation area based on microbead movement on th

SUBMITTER: Sekiya S 

PROVIDER: S-EPMC7422605 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature