Influence of Lens Fluorescence on Fluorescence Lifetime Imaging Ophthalmoscopy (FLIO) Fundus Imaging and Strategies for Its Compensation.

ABSTRACT:

Purpose

To explore the contribution of crystalline lens fluorescence to fluorescence lifetimes measured with fluorescence lifetime imaging ophthalmoscopy (FLIO) and to propose a computational model to reduce the lens influence.

Methods

FLIO, which detects autofluorescence decay over time in a short-wavelength spectral channel (SSC, 498-560 nm) and a long-wavelength spectral channel (LSC, 560-720 nm), was performed on 32 patients before and after cataract extraction. The mean autofluorescence lifetime (τ _m ) of the fundus was determined from a three-exponential fit of the postoperative fluorescence decays. The preoperative measurements were fit with series of exponential functions in which one fluorescence component was time-shifted in order to represent len

SUBMITTER: Brauer JL 

PROVIDER: S-EPMC7422756 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature