Characterisation of Glutathione Export from Human Donor Lenses.

ABSTRACT:

Purpose

To investigate whether human donor lenses are capable of exporting reduced glutathione.

Methods

Human lenses of varying ages were cultured in artificial aqueous humor for 1 hour under hypoxic conditions to mimic the physiologic environment and reduced glutathione (GSH) and oxidized glutathione (GSSG) levels measured in the media and in the lens.

Results

Human donor lenses released both GSH and GSSG into the media. Donor lenses cultured in the presence of acivicin, a γ-glutamyltranspeptidase inhibitor, exhibited a significant increase in GSSG levels (P < 0.05), indicating that GSSG undergoes degradation into its constituent amino acids. Screening of GSH/GSSG efflux transporters revealed Mrp1, Mrp4, and Mrp5 to be present at the transcript level, but only

SUBMITTER: Li B 

PROVIDER: S-EPMC7422761 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature