Arginine as an Enhancer in Rose Bengal Photosensitized Corneal Crosslinking.

ABSTRACT:

Purpose

Oxygen-independent cornea crosslinking (CXL) using rose bengal (RB) and green light may have unique clinical applications. These studies were designed to gain insight into the arginine (arg)-enhanced anaerobic crosslinking process, to maximize crosslinking efficiency, and to test a clinically feasible method for oxygen-free CXL.

Methods

Rabbit corneas were treated ex vivo using 1 mM RB and 532 nm light. RB photodecomposition, monitored by absorption spectrophotometry, was used to optimize arg concentration and to develop an irradiation and re-dying protocol. The minimal effective green light fluence was identified by linear tensile strength measurements. RB penetration into the stroma was determined by fluorescence microscopy. To favor the anaerobic pathway, a contac

SUBMITTER: Wertheimer CM 

PROVIDER: S-EPMC7422776 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature