Dataset Information
Antimycotic Activity of Ozonized Oil in Liposome Eye Drops against Candida spp.
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ABSTRACT: Purpose
This study aims to investigate the antifungal activity and mechanism of action of ozonized oil eye drops in liposomes (Ozodrop), commercialized as eye lubricant for the treatment of dry eye syndrome and eye inflammation. The activity was tested against four clinical Candida species: C albicans, C glabrata, C krusei, and C orthopsilosis.Methods
The antifungal activity of the eye drop solution was ascertained by microdilution method in accordance with EUCAST obtaining the minimum inhibitory concentration for Ozodrop. The mechanism of action was further investigated in C albicans by measuring cell vitality, intracellular reactive oxygen species production, levels of cellular and mitochondrial (∆Ψm) membrane potential, and the extent of membrane lipid peroxidation.Results
All Candida isolates were susceptible to Ozodrop with minimum inhibitory concentration values ranging from 0.195% (v/v) for C glabrata to 6.25% (v/v) for C orthopsilosis. After 1 hour of exposure at the minimum inhibitory concentration value about 30% of cells were killed, reaching about 70% at the highest Ozodrop value. After Ozodrop exposure, C albicans showed cell membrane depolarization, increased levels of lipid peroxidation, depolarized ∆Ψm, and increased reactive oxygen species generation.Conclusions
The significant increases in reactive oxygen species production cause the accumulation of reactive oxygen species-associated damages leading to progressive Candida cell dysfunction.Translational relevance
The antifungal activity of Ozodrop was demonstrated at concentrations several times lower than the concentration that can be retrieved in ocular surface after its application. The antifungal activity of the eye drops Ozodrop would represent an interesting off-label indication for a product basically conceived as an eye lubricant.
SUBMITTER: Celenza G
PROVIDER: S-EPMC7422790 | biostudies-literature | 2020 Jul
REPOSITORIES: biostudies-literature
Publications
Antimycotic Activity of Ozonized Oil in Liposome Eye Drops against <i>Candida</i> spp.
Translational vision science & technology 20200702 8
<h4>Purpose</h4>This study aims to investigate the antifungal activity and mechanism of action of ozonized oil eye drops in liposomes (Ozodrop), commercialized as eye lubricant for the treatment of dry eye syndrome and eye inflammation. The activity was tested against four clinical <i>Candida</i> species: <i>C</i><i>albicans,</i><i>C</i><i>glabrata,</i><i>C</i><i>krusei</i>, and <i>C</i><i>orthopsilosis</i>.<h4>Methods</h4>The antifungal activity of the eye drop solution was ascertained by micro ...[more]
