Relative quantification of BCL2 mRNA for diagnostic usage needs stable uncontrolled genes as reference.

ABSTRACT: Dysregulation of BCL2 is a pathophysiology observed in haematological malignancies. For implementation of available treatment-options it is preferred to know the relative quantification of BCL2 mRNA with appropriate reference genes. For the choice of reference genes-(i) Reference Genes were selected by assessing variation of >60,000 genes from 4 RNA-seq datasets of haematological malignancies followed by filtering based on their GO biological process annotations and proximity of their chromosomal locations to known disease translocations. Selected genes were experimentally validated across various haematological malignancy samples followed by stability comparison using geNorm, NormFinder, BestKeeper and RefFinder. (ii) 43 commonly used Reference Genes were obtained from literature through

SUBMITTER: Dwivedi N 

PROVIDER: S-EPMC7423076 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature