A flexible format LAMP assay for rapid detection of Ebola virus.

ABSTRACT:

Background

The unprecedented 2013/16 outbreak of Zaire ebolavirus (Ebola virus) in West Africa has highighted the need for rapid, high-throughput and POC diagnostic assays to enable timely detection and appropriate triaging of Ebola Virus Disease (EVD) patients. Ebola virus is highly infectious and prompt diagnosis and triage is crucial in preventing further spread within community and healthcare settings. Moreover, due to the ecology of Ebola virus it is important that newly developed diagnostic assays are suitable for use in both the healthcare environment and low resource rural locations.

Methodology/principle findings

A LAMP assay was successfully developed with three detection formats; a real-time intercalating dye-based assay, a real-time probe-based assay to enable mu

SUBMITTER: Bonney LC 

PROVIDER: S-EPMC7423149 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature