Extracellular vesicle-encapsulated IL-10 as novel nanotherapeutics against ischemic AKI.

ABSTRACT: Recently, extracellular vesicles (EVs) have been attracting strong research interest for use as natural drug delivery systems. We report an approach to manufacturing interleukin-10 (IL-10)-loaded EVs (IL-10⁺ EVs) by engineering macrophages for treating ischemic acute kidney injury (AKI). Delivery of IL-10 via EVs enhanced not only the stability of IL-10, but also its targeting to the kidney due to the adhesive components on the EV surface. Treatment with IL-10⁺ EVs significantly ameliorated renal tubular injury and inflammation caused by ischemia/reperfusion injury, and potently prevented the transition to chronic kidney disease. Mechanistically, IL-10⁺ EVs targeted tubular epithelial cells, and suppressed mammalian target of rapamycin signaling, thereby pr

SUBMITTER: Tang TT 

PROVIDER: S-EPMC7423360 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature