Serial in-solution digestion protocol for mass spectrometry-based glycomics and proteomics analysis.

ABSTRACT: Advancement in mass spectrometry has revolutionized the field of proteomics. However, there remains a gap in the analysis of protein post-translational modifications (PTMs), particularly for glycosylation. Glycosylation, the most common form of PTM, is involved in most biological processes; thus, analysis of glycans along with proteins is crucial to answering important biologically relevant questions. Of particular interest is the brain extracellular matrix (ECM), which has been called the "final Frontier" in neuroscience, which consists of highly glycosylated proteins. Among these, proteoglycans (PGs) contain large glycan structures called glycosaminoglycans (GAGs) that form crucial ECM components, including perineuronal nets (PNNs), shown to be altered in neuropsychiatric diseases. Thus,

SUBMITTER: Sethi MK 

PROVIDER: S-EPMC7423595 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature