Whole-body tracking of single cells via positron emission tomography.

ABSTRACT: In vivo molecular imaging can measure the average kinetics and movement routes of injected cells through the body. However, owing to non-specific accumulation of the contrast agent and its efflux from the cells, most of these imaging methods inaccurately estimate the distribution of the cells. Here, we show that single human breast cancer cells loaded with mesoporous silica nanoparticles concentrating the ⁶⁸Ga radioisotope and injected into immunodeficient mice can be tracked in real time from the pattern of annihilation photons detected using positron emission tomography, with respect to anatomical landmarks derived from X-ray computed tomography. The cells travelled at an average velocity of 50 mm s^-1 and arrested in the lungs 2-3 s after tail-vein injection into th

SUBMITTER: Jung KO 

PROVIDER: S-EPMC7423763 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature