Regulation of senescence-associated protease genes by sulphur availability according to barley (Hordeum vulgare L.) phenological stage.

ABSTRACT:

Background and aims

Proteases are responsible for protein degradation during leaf senescence, allowing nutrients to be redirected to sink tissues. In a previous work, we reported that sulphur deficiency produced a delay in the leaf senescence of barley (Hordeum vulgare L.) plants, at both vegetative and reproductive stages. In this work, we analyse the effect of sulphur deficiency on the expression of several genes coding for proteases of different catalytic groups, which have been strongly associated with leaf senescence.

Methods

Four independent experiments were performed in order to impose low sulphur availability conditions: one of steady-state sulphur deficiency during the vegetative stage and three of sulphur starvation during vegetative and reproductive stages.

Key

SUBMITTER: Veliz CG 

PROVIDER: S-EPMC7424724 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature