Reparative effect of mesenchymal stromal cells on endothelial cells after hypoxic and inflammatory injury.

ABSTRACT:

Background

The renal endothelium is a prime target for ischemia-reperfusion injury (IRI) during donation and transplantation procedures. Mesenchymal stromal cells (MSC) have been shown to ameliorate kidney function after IRI. However, whether this involves repair of the endothelium is not clear. Therefore, our objective is to study potential regenerative effects of MSC on injured endothelial cells and to identify the molecular mechanisms involved.

Methods

Human umbilical vein endothelial cells (HUVEC) were submitted to hypoxia and reoxygenation and TNF-α treatment. To determine whether physical interaction or soluble factors released by MSC were responsible for the potential regenerative effects of MSC on endothelial cells, dose-response experiments were performed in co-cult

SUBMITTER: Sierra-Parraga JM 

PROVIDER: S-EPMC7424997 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature