Genetic diversity of group A rotavirus in acute gastroenteritis outpatients in Shanghai from 2017 to 2018.

ABSTRACT: BACKGROUND:Group A Rotavirus (RVA), despite being an important pathogen in hospitalized children, is less studied in pediatric outpatients, and even rarely investigated in adults. This study aims to understand the genetic diversity of RVA in outpatients across all age groups in Shanghai, and thus providing a molecular basis for vaccine implementation and evaluation. METHODS:Stool samples were first screened by Real-time Reverse Transcription Polymerase Chain Reaction (rRT-PCR). RVA genotyping was performed through the amplification of partial VP7 and VP4 gene. Strains of interest were further sequenced and analyzed using MEGA 6.0. RESULTS:Four thousand nine hundred one samples were collected, from which 7.61% (373 cases) were screened positive for RVA. RVA prevalence was higher in children

SUBMITTER: Kuang X 

PROVIDER: S-EPMC7425067 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature