Effective Method for Accurate and Sensitive Quantitation of Rapid Changes of Newly Synthesized Proteins.

ABSTRACT: Protein synthesis is quickly and tightly regulated in cells to adapt to the ever-changing extracellular and intracellular environment. Accurate quantitation of rapid protein synthesis changes can provide insights into protein functions and cellular activities, but it is very challenging to achieve because of the lack of effective analysis methods. Here, we developed an effective mass spectrometry-based method named quantitative O-propargyl-puromycin tagging (QOT) by integrating O-propargyl-puromycin (OPP) labeling, bioorthogonal chemistry, and multiplexed proteomics for global and quantitative analysis of rapid protein synthesis. The current method enables us to accurately quantitate rapid changes of newly synthesized proteins because, unlike amino acids and their analogs, OPP can be utili

SUBMITTER: Tong M 

PROVIDER: S-EPMC7425198 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature