High-resolution epitope mapping of anti-Hu and anti-Yo autoimmunity by programmable phage display.

ABSTRACT: Paraneoplastic neurological disorders are immune-mediated diseases understood to manifest as part of a misdirected anti-tumor immune response. Paraneoplastic neurological disorder-associated autoantibodies can assist with diagnosis and enhance our understanding of tumor-associated immune processes. We designed a comprehensive library of 49-amino-acid overlapping peptides spanning the entire human proteome, including all splicing isoforms and computationally predicted coding regions. Using this library, we optimized a phage immunoprecipitation and sequencing protocol with multiple rounds of enrichment to create high-resolution epitope profiles in serum and cerebrospinal fluid (CSF) samples from patients suffering from two common paraneoplastic neurological disorders, the anti-Yo (n =

SUBMITTER: O'Donovan B 

PROVIDER: S-EPMC7425417 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature