Cumulative contact frequency of a chromatin region is an intrinsic property linked to its function.

ABSTRACT: Regulation of gene transcription is a complex process controlled by many factors, including the conformation of chromatin in the nucleus. Insights into chromatin conformation on both local and global scales can be provided by the Hi-C (high-throughput chromosomes conformation capture) method. One of the drawbacks of Hi-C analysis and interpretation is the presence of systematic biases, such as different accessibility to enzymes, amplification, and mappability of DNA regions, which all result in different visibility of the regions. Iterative correction (IC) is one of the most popular techniques developed for the elimination of these systematic biases. IC is based on the assumption that all chromatin regions have an equal number of observed contacts in Hi-C. In other words, the IC procedure

SUBMITTER: Samborskaia MD 

PROVIDER: S-EPMC7425636 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature