Hypermethylation of the IRAK3-Activated MAPK Signaling Pathway to Promote the Development of Glioma.

ABSTRACT:

Objective

This study aimed to elucidate the molecular mechanism underlying the involvement of abnormal DNA methylation in the development of glioma and identify potential new targets for glioma therapy.

Methods

The GSE79122 chip achieved from the Gene Expression Omnibus (GEO) database containing 69 glioma samples and 9 normal samples was analyzed. Methylation-specific polymerase chain reaction (MS-PCR or MSP), reverse transcription-PCR, and Western blot analysis were used to confirm the methylation level and expression level of the interleukin receptor-associated kinase (IRAK3) gene in glioma cells, 36 glioma samples, and the corresponding normal samples. In vitro, the proliferation, apoptosis rate, migration, and invasion abilities of glioma cells were detected by Ce

SUBMITTER: Wu X 

PROVIDER: S-EPMC7425661 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature