Excess Transforming Growth Factor-α Changed the Cell Properties of Corneal Epithelium and Stroma.

ABSTRACT:

Purpose

This study is to investigate the corneal anomaly caused by excess transforming growth factor-α (TGF-α) during mouse development.

Methods

Bitransgenic KeraRT/TGF-α mice, generated via cross-mating tetO-TGF-α and KeraRT mice, were induced to overexpress TGF-α by doxycycline commencing at embryonic day 0 or postnatal day 0 to different developmental stages. Bitransgenic mice with doxycycline induction were defined as TGF-αECK mice (TGF-α excess expression by corneal keratocytes). Mouse eyes were examined by hematoxylin and eosin staining, immunofluorescent staining and transmission electron microscopy. Protein and RNA from mouse cornea were subjected to western blotting and real-time quantitative polymerase chain reaction.

Results

In TGF-αECK mice, TGF-α overexpr

SUBMITTER: Zhang L 

PROVIDER: S-EPMC7425719 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature