Retinoic Acid Potentiates Orbital Tissues for Inflammation Through NF-κB and MCP-1.

ABSTRACT:

Purpose

The orbit displays unique vulnerability to inflammatory conditions. The most prevalent of these conditions, thyroid eye disease (TED), occurs in up to 50% of patients with Graves' disease (GD). Whereas the pathology of both TED and GD is driven by autoantibodies, it is unclear why symptoms manifest specifically in the orbit.

Methods

We performed retinoic acid treatment on both normal and TED patient-derived orbital fibroblasts (OFs) followed by mRNA and protein isolation, quantitative real-time polymerase chain reaction (qRT-PCR), enzyme-linked immunosorbent assay, RNA sequencing, and Western blot analyses.

Results

Both normal and TED patient-derived OFs display robust induction of monocyte chemoattractant protein 1 (MCP-1) upon retinoid treatment; TED OFs sec

SUBMITTER: Unsworth SP 

PROVIDER: S-EPMC7425727 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature